Badaruddin R H, Waldrop F S, Puchtler H
Arch Pathol Lab Med. 1976 Nov;100(11):616-9.
Previous histochemical studies demonstrated a myoid layer along biliary pathways. This report describes alterations of the pericanalicular layer in various lesions. Carnoy- or methacarn-fixed sections of livers from 200 autopsies were treated with the tannic acid-phosphomolybdic acid-dye method for myosins. In other series, methods for myosins were combined with the picro-Sirius Red F3BA stain or the Prussian Blue reaction. In normal liver, bile canaliculi were lined by a narrow myoid layer. In other cases, eg, hepatic cirrhosis, the pericanalicular layer showed slight to moderate thickening. In cases with obstruction or atresia of bile ducts, the dilated canaliculi were surrounded by a prominent myoid layer. These observations support theories that the pericanalicular layer is contractile and promotes bile flow under positive pressure.
以往的组织化学研究表明，沿胆管途径存在肌样层。本报告描述了不同病变中胆小管周围层的改变。对200例尸检肝脏经卡诺氏液或甲醇-氯仿固定的切片，采用鞣酸-磷钼酸-染料法检测肌球蛋白。在其他系列研究中，检测肌球蛋白的方法与苦味酸-天狼星红F3BA染色或普鲁士蓝反应相结合。在正常肝脏中，胆小管内衬有一层狭窄的肌样层。在其他情况下，如肝硬化，胆小管周围层显示轻度至中度增厚。在胆管梗阻或闭锁的病例中，扩张的胆小管被一层明显的肌样层所包围。这些观察结果支持了以下理论，即胆小管周围层具有收缩性，并在正压下促进胆汁流动。
