McNeal E T, Creveling C R, Daly J W
J Neurochem. 1980 Aug;35(2):338-42. doi: 10.1111/j.1471-4159.1980.tb06269.x.
The accumulations of radioactive cyclic AMP elicited by adenosine, norepinephrine, and histamine in adenine-labeled vesicular entities of a particulate fraction from guinea pig cerebral cortex are greatly reduced as a result of prolonged preincubation. The presence of adenosine deaminase during preincubations largely prevents the loss of adenosine, norepinephrine and histamine responses. Adenosine deaminase was inactivated by deoxycoformycin prior to stimulation of cyclic AMP accumulation by adenosine or amines. If adenosine deaminase is not inactivated, responses to norepinephrine are not significant and histamine responses are reduced by 50%. Adenosine deaminase cannot restore responsiveness of the cyclic AMP-generating systems. It is proposed that, in particulate fractions of guinea pig cerebral cortex, low levels of adenosine cause a slow loss of receptors and/or coupling of receptors to cyclic AMP-generating systems.
由于长时间预孵育，豚鼠大脑皮层微粒部分腺嘌呤标记的囊泡实体中，由腺苷、去甲肾上腺素和组胺引发的放射性环磷酸腺苷积累量大幅减少。预孵育期间腺苷脱氨酶的存在很大程度上防止了腺苷、去甲肾上腺素和组胺反应的丧失。在通过腺苷或胺刺激环磷酸腺苷积累之前，脱氧助间型霉素使腺苷脱氨酶失活。如果腺苷脱氨酶未失活，对去甲肾上腺素的反应不显著，对组胺的反应减少50%。腺苷脱氨酶无法恢复环磷酸腺苷生成系统的反应性。有人提出，在豚鼠大脑皮层微粒部分中，低水平的腺苷会导致受体缓慢丧失和/或受体与环磷酸腺苷生成系统的偶联丧失。
