Salvaterra P, Matthews D A, Foders R
J Neurochem. 1980 Nov;35(5):1253-7. doi: 10.1111/j.1471-4159.1980.tb07885.x.
The hippocampus is well suited for studies of the interrelationships of various neurotransmitter systems in the CNS by reason of its simple laminated organization, defined connections, and variety of identified neurotransmitters. We have studied the biochemical and pharmacological properties of five radiolabeled ligand binding sites in a membrane fraction prepared from rat hippocampal formation. These binding sites are thought to identify recognition sites for neurotransmitter receptors. The rank order of ligand binding sites is [3H]muscimol > [3H]quinuclidinyl benzilate > [3H]dihydroergocryptine > [3H]dihydroalprenolol > 125I-labeled alpha-bungarotoxin. All ligands have a single, saturable, high-affinity binding site. Pharmacological characterization of the ligand binding sites indicates properties consistent with the identification of these sites as neurotransmitter receptors.
由于海马体具有简单的分层结构、明确的连接以及多种已确定的神经递质，因此它非常适合用于研究中枢神经系统中各种神经递质系统的相互关系。我们已经研究了从大鼠海马结构制备的膜组分中五个放射性标记配体结合位点的生化和药理学特性。这些结合位点被认为可识别神经递质受体的识别位点。配体结合位点的排序为[3H]蝇蕈醇>[3H]东莨菪碱>[3H]双氢麦角隐亭>[3H]双氢心得舒>125I标记的α-银环蛇毒素。所有配体都有一个单一的、可饱和的、高亲和力结合位点。配体结合位点的药理学特征表明这些位点被识别为神经递质受体的特性是一致的。
