Miwa S, Inagaki C, Fujiwara M, Takaori S
Jpn J Pharmacol. 1980 Jun;30(3):337-45. doi: 10.1254/jjp.30.337.
Properties of adenosine triphosphatases (ATPases) in the choroid plexus of rabbit cerebral ventricles were investigated using tissue homogenates and subcellular fractions. Na,K-ATPase or HCO3-ATPase activity of choroid plexus was significantly lower than that of the brain or the kidney, respectively. Among the homogenates of choroid plexus from the lateral, third and fourth ventricles, there were no differences in the activities of Na,K-, Mg- and HCO3-ATPases. In the choroid plexus, Na,K-ATPase activity was demonstrated to be highest in 10,000 X g fraction, while the highest Mg- and HCO3-ATPase activities were observed in 8,000 X g fraction, the so-called mitochondrial fraction. Ion requirement, pH optimum and ouabain sensitivity of Na,K-ATPase in 10,000 X g fraction of choroid plexus were similar to findings in erythrocytes, brain and kidney. However, properties of Mg- and HCO3-ATPases in 8,000 X g fraction were considerably different from those found in the brain and gastric mucosa. In the choroid plexus, thiocyanate ion inhibited Mg-ATPase as well as HCO3-ATPase. These results indicate that characteristics of ATPase systems in the choroid plexus are to some extent different from those in tissues such as brain, kidney and gastric mucosa.
利用组织匀浆和亚细胞组分，对兔脑室脉络丛中三磷酸腺苷酶（ATP酶）的特性进行了研究。脉络丛的钠钾ATP酶或碳酸氢根ATP酶活性分别显著低于脑或肾的相应酶活性。在侧脑室、第三脑室和第四脑室脉络丛的匀浆中，钠钾、镁和碳酸氢根ATP酶的活性没有差异。在脉络丛中，钠钾ATP酶活性在10,000×g组分中最高，而镁和碳酸氢根ATP酶的最高活性则在8,000×g组分（即所谓的线粒体组分）中观察到。脉络丛10,000×g组分中钠钾ATP酶的离子需求、最适pH值和哇巴因敏感性与红细胞、脑和肾中的发现相似。然而，8,000×g组分中镁和碳酸氢根ATP酶的特性与脑和胃黏膜中的特性有很大不同。在脉络丛中，硫氰酸根离子抑制镁ATP酶以及碳酸氢根ATP酶。这些结果表明，脉络丛中ATP酶系统的特性在一定程度上不同于脑、肾和胃黏膜等组织中的特性。
