Lopez D M, Sigel M M, Charyulu V L
J Natl Cancer Inst. 1981 Jan;66(1):191-6.
Spleen cells from BALB/cCrgl mice responded to murine mammary tumor virus (MuMTV) in cell-mediated immune assays at higher levels than did the spleen cells from the syngeneic BALB/cfC3H mice. Implantation in BALB/cCrgl with a chemically induced mammary tumor and in BALB/cfCrgl mice with spontaneous mammary tumors (SMT) arising in the same strain resulted in sensitization of these animals to the antigens of their tumors. Reactivities peaked 3 weeks after transplantation, whereas no positive reactions could be detected when tumors reached maximum size. A kinetic study with the use of MuMTV antigen(s) showed that the responses of lymphocytes from BALB/cfC3H with SMT followed the same pattern as that obtained with tumor antigens, which indicated that this might be a de novo sensitization. In sharp contrast, a steady type of response to MuMTV was observed with the spleen cells from BALB/cCrgl mice; i.e., the levels of responsiveness to MuMTV did not significantly vary at any stage of tumor development. In vivo studies explored the possible relevance of the in vitro cell-mediated immunity to the host defenses. MuMTV-expressing mammary tumor cells were implanted in BALB/cCrgl and BALB/cfC3H mice. The total incidence of tumors was significantly reduced and a delay occurred in their time of appearance in BALB/cCrgl mice in relation to BALB/cfC3H animals. Thus the in vitro reactivity to MuMTV antigen(s) in BALB/cCrgl mice was found to be coincidental with a degree of protection against the development of MuMTV-expressing mammary tumors.
在细胞介导的免疫分析中，BALB/cCrgl小鼠的脾细胞对鼠乳腺肿瘤病毒（MuMTV）的反应水平高于同基因的BALB/cfC3H小鼠的脾细胞。将化学诱导的乳腺肿瘤植入BALB/cCrgl小鼠，以及将同一品系中自发产生乳腺肿瘤（SMT）的肿瘤植入BALB/cfCrgl小鼠，会使这些动物对其肿瘤抗原产生致敏作用。移植后3周反应性达到峰值，而当肿瘤达到最大大小时则检测不到阳性反应。一项使用MuMTV抗原的动力学研究表明，患有SMT的BALB/cfC3H小鼠淋巴细胞的反应模式与肿瘤抗原的反应模式相同，这表明这可能是一种从头致敏。与之形成鲜明对比的是，观察到BALB/cCrgl小鼠的脾细胞对MuMTV有稳定的反应类型；即，在肿瘤发展的任何阶段，对MuMTV的反应水平都没有显著变化。体内研究探讨了体外细胞介导的免疫与宿主防御的可能相关性。将表达MuMTV的乳腺肿瘤细胞植入BALB/cCrgl和BALB/cfC3H小鼠。与BALB/cfC3H动物相比，BALB/cCrgl小鼠的肿瘤总发生率显著降低，且肿瘤出现时间延迟。因此，发现BALB/cCrgl小鼠对MuMTV抗原的体外反应性与对表达MuMTV的乳腺肿瘤发展的一定程度的保护作用相一致。
