Jakobovits E B, Saragosti S, Yaniv M, Aloni Y
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6556-60. doi: 10.1073/pnas.77.11.6556.
We have used a low-salt detergent-free extraction procedure on cells infected with simian virus 40 to obtain viral nucleoprotein late after infection. Addition of EScherichia coli RNA polymerase and ribonucleotide triphosphates to the viral minichromosomes permitted transcription of RNA from viral templates. This synthesis was initiated predominantly within a fragment of DNA spanning 0.67 to 0.76 map unit on the genome. The synthesis from this region proceeded primarily along the "late" strand in a clockwise direction. These results were in contrast to the synthesis obtained with naked viral DNA in which initiation occurred on other regions of the genome and from which transcription proceeded counterclockwise along the early strand. These findings indicate that the nucleoprotein template or factors tightly associated with it may be responsible for site(s) and strand selection in transcription of simian virus 40.
我们对感染了猿猴病毒40的细胞采用了一种低盐无去污剂提取程序，以在感染后期获得病毒核蛋白。向病毒微型染色体中添加大肠杆菌RNA聚合酶和核糖核苷酸三磷酸可使RNA从病毒模板转录。这种合成主要在基因组上跨度为0.67至0.76图距单位的一段DNA片段内起始。来自该区域的合成主要沿“晚期”链按顺时针方向进行。这些结果与用裸露病毒DNA获得的合成结果相反，在裸露病毒DNA中，起始发生在基因组的其他区域，转录沿早期链逆时针进行。这些发现表明，核蛋白模板或与其紧密相关的因子可能负责猿猴病毒40转录中的位点和链选择。
