Liteplo R G, Sribney M
Biochim Biophys Acta. 1980 Sep 8;619(3):660-8. doi: 10.1016/0005-2760(80)90115-0.
GTP has been found to markedly enhance the formation of CDPdiacylglycerol in rat liver microsomes. The magnitude of the GTP stimulatory effect was influenced by the fatty acid composition of the phosphatidic acid substrate. Heat or proteolytic treatment of microsomal membranes prevented GTP from stimulating liponucleotide formation. Treatment of microsomes with low levels of detergent reduced the stimulatory action of the GTP. The increased synthesis of CDPdiacylglycerol obtained by the addition of GTP to the incubation medium is due to an increase in CTP:phosphatidate cytidylyltransferase activity, rather than to a reduction in the breakdown of either the substrates or product of the reaction. The results suggest that some other protein or enzyme mediates the effect of GTP upon CTP:phosphatidate cytidylyltransferase activity.
已发现鸟苷三磷酸（GTP）能显著增强大鼠肝脏微粒体中CDP二酰甘油的形成。GTP刺激作用的强度受磷脂酸底物脂肪酸组成的影响。对微粒体膜进行加热或蛋白酶处理可阻止GTP刺激脂核苷酸的形成。用低水平去污剂处理微粒体可降低GTP的刺激作用。通过向孵育培养基中添加GTP而获得的CDP二酰甘油合成增加，是由于CTP：磷脂酸胞苷转移酶活性增加，而不是由于反应底物或产物分解的减少。结果表明，其他某种蛋白质或酶介导了GTP对CTP：磷脂酸胞苷转移酶活性的作用。
