McMurray W C, Jarvis E C
Can J Biochem. 1980 Oct;58(10):771-6. doi: 10.1139/o80-108.
The enzyme responsible for the conversion of phosphatidylglycerol to diphosphatidylglycerol (cardiolipin) in the presence of cytidine diphosphate diacylglycerol is firmly associated with mitochondrial membranes and is not extracted with hypotonic or hypertonic media or with nonionic detergents. Some solubilization was obtained with bile salt solutions, but the zwitter-ionic detergent. Miranol H2M, was most effective in extracting the enzyme. The Miranol extracts were fractionated by column chromatography on Bio-Gel A-1.5 m. The solubilized enzyme is considerably more active in converting unsaturated than saturated phosphatidyl-glycerols, but shows little preference for the cytidine diphosphate diacylglycerols with different fatty acyl substituents. There is an absolute dependence upon divalent cations with the order of effectiveness: Co2+ much greater than Mn2+ greater than Mg2+. In the presence of optimal levels of Co2+ other divalent cations are inhibitory with the order of inhibition: Cd2+ greater than Zn2+ greater than Ca2+ greater than Ba2+ greater than Cu2+ greater than Hg2+ greater than Ni2+. The solubilized enzyme exhibited no requirement for added phospholipids and several phospholipids inhibited the reaction in the order: diphosphatidylglycerol greater than phosphatidylethanolamine greater than phosphatidylserine greater than phosphatidylinositol.
在胞苷二磷酸二酰基甘油存在的情况下，负责将磷脂酰甘油转化为二磷脂酰甘油（心磷脂）的酶与线粒体膜紧密结合，不能被低渗或高渗介质或非离子去污剂提取。用胆汁盐溶液可实现部分溶解，但两性离子去污剂米腊诺尔H2M在提取该酶方面最有效。米腊诺尔提取物通过在生物凝胶A - 1.5m上进行柱色谱分离。溶解的酶在转化不饱和磷脂酰甘油方面比饱和磷脂酰甘油的活性高得多，但对具有不同脂肪酰基取代基的胞苷二磷酸二酰基甘油几乎没有偏好。绝对依赖二价阳离子，其有效性顺序为：Co2+远大于Mn2+大于Mg2+。在Co2+达到最佳水平时，其他二价阳离子具有抑制作用，抑制顺序为：Cd2+大于Zn2+大于Ca2+大于Ba2+大于Cu2+大于Hg2+大于Ni2+。溶解的酶不需要添加磷脂，几种磷脂按以下顺序抑制反应：二磷脂酰甘油大于磷脂酰乙醇胺大于磷脂酰丝氨酸大于磷脂酰肌醇。
