Chan Y K, Nelson L M, Knowles R
Can J Microbiol. 1980 Sep;26(9):1126-31. doi: 10.1139/m80-186.
Production of H2 by Azospirillum brasilense under N2-fixing conditions was studied in continuous and batch cultures. Net H2 production was consistently observed only when the gas phase contained CO. Nitrogenase activity (C2H2 reduction) and H2 evolution (in the presence of 5% CO) showed a similar response to O2 and were highest at 0.75% dissolved O2. Uptake hydrogenase activity, ranging from 0.3 to 2.5 mumol H2/mg protein per hour was observed in batch cultures under N2. Such rates were more than sufficient to recycle nitrogenase-produced H2. Tritium-exchange assay showed that H2 uptake was higher under Ar than under N2. Uptake hydrogenase was strongly inhibited by CO and C2H2. Cyclic GMP inhibited both nitrogenase and uptake hydrogenase activities.
在连续培养和分批培养条件下，研究了巴西固氮螺菌在固氮条件下氢气的产生情况。只有当气相中含有一氧化碳时，才持续观察到净氢气产生。固氮酶活性（乙炔还原）和氢气释放（在5%一氧化碳存在下）对氧气表现出相似的响应，并且在溶解氧为0.75%时最高。在氮气条件下的分批培养中，观察到吸氢酶活性为每小时0.3至2.5微摩尔氢气/毫克蛋白质。这样的速率足以回收固氮酶产生的氢气。氚交换试验表明，在氩气条件下的氢气吸收高于氮气条件下。吸氢酶受到一氧化碳和乙炔的强烈抑制。环鸟苷酸抑制固氮酶和吸氢酶的活性。
