劳氏肉瘤病毒长末端重复序列中功能性启动子的鉴定
Identification of a functional promoter in the long terminal repeat of Rous sarcoma virus.
作者信息
Yamamoto T, de Crombrugghe B, Pastan I
出版信息
Cell. 1980 Dec;22(3):787-97. doi: 10.1016/0092-8674(80)90555-3.
PMID:6257399
Abstract
Specific initiation of transcription of Rous sarcoma virus by RNA polymerase II was obtained in a cell-free system using cloned RSV DNA as template. The site of initiation is located in the common "C" region of the long terminal repeat (LTR), 23 bp downstream from the promoter-like sequence TATTTAAG. This finding indicates that the basic information necessary for RSV transcription lies within the viral genome.
摘要
在一个无细胞系统中，以克隆的劳斯肉瘤病毒（RSV）DNA为模板，实现了RNA聚合酶II对劳斯肉瘤病毒转录的特异性起始。起始位点位于长末端重复序列（LTR）的共同“C”区域，在类似启动子序列TATTTAAG下游23个碱基对处。这一发现表明，RSV转录所需的基本信息存在于病毒基因组内。
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