Maurel E, Bouissou H, Pieraggi M T, Julian M
Connect Tissue Res. 1980;8(1):33-9. doi: 10.3109/03008208009152119.
A biochemical study of skin biopsies of young normal subjects and two groups of aged subjects, one active and the other confined to bed, was made and the results compared with histological observations. Skin biopsies were incubated with 3H proline and 14C glucosamine and connective tissue components were sequentially extracted. In each extract the specific activity of total and collagenous protein was determined. With age a decrease was observed in both the amount of protein and the 3H proline incorporation of collagenous extracts. A parallel increase in 14C glucosamine incorporation was also noticed in extracts containing glycosaminoglycans and structural proteins. These results agree in part with the changes observed by light and electron microscopy, which revealed an increase in ground substance and fragmentation of collagen bundles in the dermis of older subjects.
对年轻正常受试者以及两组老年受试者（一组活动自如，另一组卧床不起）的皮肤活检标本进行了生化研究，并将结果与组织学观察结果进行了比较。将皮肤活检标本与3H脯氨酸和14C葡糖胺一起孵育，然后依次提取结缔组织成分。在每种提取物中测定总蛋白和胶原蛋白的比活性。随着年龄的增长，胶原蛋白提取物中的蛋白质含量和3H脯氨酸掺入量均下降。在含有糖胺聚糖和结构蛋白的提取物中，还注意到14C葡糖胺掺入量平行增加。这些结果部分与光学显微镜和电子显微镜观察到的变化一致，后者显示老年受试者真皮中基质增加，胶原束断裂。
