Koval' V G, Morozova R P
Ukr Biokhim Zh (1978). 1978 Jan-Feb;50(1):64-71.
The ergosterol and lecithin absorption IR-spectra were studied in a nonpolar anhydrous medium. The thermodynamical and spectral characteristics of dimeric associates and the enthalpy value for trimeric associates of this sterol are determined. Thermodynamical and spectral parameters of ergosterol intermolecular associates with lecithin in a nonpolar anhydrous medium are found. It is established that the intermolecular interaction of lecithin with ergosterol occurs according to the mechanism of hydrogen bond. A conclusion is drawn that the presence of binary bonds and methyl groups in the cyclic and aliphatic parts of the sterol molecule affects greatly the structure of the model membrane and its strength. It is shown that under conditions of the experiment the oxygen of the phosphate group contributes to formation of the molecular associates of lecithin with sterols and not that of the carbonyl group. The obtained experimental data may be at use when studying structural disturbances of native membranes in norm and with different pathologies.
在非极性无水介质中研究了麦角甾醇和卵磷脂的吸收红外光谱。测定了二聚体缔合物的热力学和光谱特征以及该甾醇三聚体缔合物的焓值。发现了麦角甾醇与卵磷脂在非极性无水介质中分子间缔合物的热力学和光谱参数。确定了卵磷脂与麦角甾醇的分子间相互作用是按照氢键机制发生的。得出结论，甾醇分子环状和脂肪族部分中的二元键和甲基的存在极大地影响了模型膜的结构及其强度。结果表明，在实验条件下，磷酸基团的氧有助于卵磷脂与甾醇形成分子缔合物，而不是羰基的氧。所获得的实验数据在研究正常和不同病理状态下天然膜的结构紊乱时可能会有用。
