[Isolation of cytopathogenic strains of infectious bursal virus of chickens in cell cultures].

Five virus strains with cytopathogenic properties for the cell cultures of chick embryo fibroblasts and embryo kidney cells were isolated from chickens clinically suspected of suffering from infectious bursitis. According to the form of cytopathic effect (CPE) on cell cultures, according to chloroform and thermal resistance, nature of nucleic acid (RNA), according to the absence of the production of intracellular inclusions, negative haemagglutination, and according to antigenic identity with the reference strain, the isolates were deemed to belong to the viruses of infectious bursitis. This conclusion was also corroborated by histological and serological studies in isolates of infected experimental animals.