Triner L, Vulliemoz Y, Verosky M, Alpert M
Anesthesiology. 1981 Mar;54(3):193-8. doi: 10.1097/00000542-198103000-00004.
The effect of halothane on cerebellar control of motor activity and on cerebellar cyclic 3',5'-guanosine monophosphate (cGMP) content was studied in mice. Isoniazide and picrotoxin were used to increase motor activity and induce seizures associated with an increase in cerebellar cGMP content. Halothane markedly decreased the cerebellar cGMP content (by 60 per cent at 0.61 per cent, the concentration at which 50 per cent of mice lost righting reflex) and prevented the isoniazide-induced increase in cGMP content. Halothane, 0.61 per cent, significantly reduced both isoniazide- and picrotoxin-induced motor activity; the ED50 convulsive dose of isoniazide (137.7 +/- 7.04) and of picrotoxin (1.9 +/- 0.2 mg x kg-1, sc) was about three times higher (402.2 +/- 17.9 and 5.8 +/- 0.6 mg x kg-1, sc, respectively) in mice exposed to halothane. In contrast, halothane did not alter the ED50 convulsive dose of strychnine, which has a different site and mechanism of action, blockade of glycine receptors, a mechanism not involving the cerebellar system. These results indicate that halothane has a significant effect on the cerebellar control of motor activity and that cGMP plays an important role in the alteration of cerebellar function by halothane.
研究了氟烷对小鼠小脑运动活动控制及小脑环磷鸟苷（cGMP）含量的影响。使用异烟肼和印防己毒素来增加运动活动并诱发与小脑cGMP含量增加相关的癫痫发作。氟烷显著降低了小脑cGMP含量（在0.61%浓度时降低60%，该浓度下50%的小鼠失去翻正反射），并阻止了异烟肼诱导的cGMP含量增加。0.61%的氟烷显著降低了异烟肼和印防己毒素诱导的运动活动；在接触氟烷的小鼠中，异烟肼（137.7±7.04）和印防己毒素（1.9±0.2mg·kg-1，皮下注射）的ED50惊厥剂量分别约高出三倍（分别为402.2±17.9和5.8±0.6mg·kg-1，皮下注射）。相比之下，氟烷并未改变士的宁的ED50惊厥剂量，士的宁具有不同的作用位点和作用机制，即阻断甘氨酸受体，这一机制不涉及小脑系统。这些结果表明，氟烷对小脑运动活动控制有显著影响，且cGMP在氟烷改变小脑功能过程中起重要作用。
