Fattoretti P, Viticchi C, Cognini G, Gentile S, Piantanelli L
Boll Soc Ital Biol Sper. 1980 Dec 15;56(23):2421-7.
Beta-adrenergic receptors from submandibular glands of mice were studied by equilibrium binding experiments. Due to the fact that some discrepancies were previously observed among different author data, we compared two methods for non specific binding substruction, that represents the major source of errors in such experiments. Data were obtained strongly suggesting the presence of multiple population of binding sites and/or negative cooperativity.
通过平衡结合实验对小鼠下颌下腺的β-肾上腺素能受体进行了研究。由于之前在不同作者的数据之间观察到一些差异,我们比较了两种用于非特异性结合扣除的方法,这是此类实验中主要的误差来源。获得的数据有力地表明存在多个结合位点群体和/或负协同性。