Borbe H O, Müller W E, Wollert U
Brain Res. 1981 Jan 26;205(1):131-9. doi: 10.1016/0006-8993(81)90725-3.
Saturable, specific [3H]strychnine binding can be demonstrated in homogenates of bovine retina. Scatchard plots revealed only one set of binding sites with a dissociation constant (Kd) of about 60 nM and a maximal number of binding sites of about 1.5 pmol/mg protein. The structural specificity of [3H]strychnine binding sites in bovine retina parallels the properties found for [3H]strychnine binding sites in the spinal cord of several vertebrates. Thus, the data do not give any evidence that specific [3H]strychnine binding in bovine retina labels taurine rather than glycine receptors and favors glycine rather than taurine as inhibitory neurotransmitter in bovine retina. The subcellular distribution of specific [3H]strychnine binding in bovine retina parallels that of sodium-dependent, high-affinity uptake of glycine and taurine. All 3 parameters are mainly found in the P2 fractions of bovine retina homogenates, containing conventional synaptosomes, most abundant in the inner plexiform layer, but can also be found in the P1 fractions, containing large synaptosomes from the photoreceptor cell layer.
在牛视网膜匀浆中可证明存在饱和、特异性的[3H]士的宁结合。Scatchard图显示只有一组结合位点，解离常数（Kd）约为60 nM，最大结合位点数约为1.5 pmol/mg蛋白质。牛视网膜中[3H]士的宁结合位点的结构特异性与几种脊椎动物脊髓中[3H]士的宁结合位点的特性相似。因此，这些数据没有提供任何证据表明牛视网膜中特异性的[3H]士的宁结合标记的是牛磺酸受体而非甘氨酸受体，并且支持甘氨酸而非牛磺酸作为牛视网膜中的抑制性神经递质。牛视网膜中特异性[3H]士的宁结合的亚细胞分布与甘氨酸和牛磺酸的钠依赖性高亲和力摄取的分布相似。所有这三个参数主要存在于牛视网膜匀浆的P2组分中，该组分含有传统的突触体，在内网状层中最为丰富，但也可在P1组分中找到，P1组分含有来自光感受器细胞层的大突触体。
