枪乌贼轴突中的钠通道通透。II：非独立性与电流-电压关系。
Sodium channel permeation in squid axons. II: Non-independence and current-voltage relations.
作者信息
Begenisich T B, Cahalan M D
出版信息
J Physiol. 1980 Oct;307:243-57. doi: 10.1113/jphysiol.1980.sp013433.
Abstract
- The concentration and voltage dependence of current through the Na channels of squid giant axons was studied. The permeant cations Na, K and ammonium were used. 2. The Na channel current at a fixed voltage saturates as the internal permeant ion concentration is increased. The half-saturation activities at 50 mV were found to be 623, 268, 161 mM for Na, NH4, and K, respectively. These Km values for Na and K appear to be voltage-dependent. 3. Instantaneous current-voltage relations were determined with two different internal Na concentrations and with Na outside and K inside. 4. A three-barrier, two-site ion permeation model previously used in describing Na channel reversal potentials is shown here to account for the Na channel currents as well.
摘要
- 研究了枪乌贼巨大轴突钠通道中电流的浓度和电压依赖性。使用了渗透性阳离子钠、钾和铵。2. 当内部渗透性离子浓度增加时，固定电压下的钠通道电流会饱和。在50 mV时，钠、铵和钾的半饱和活性分别为623、268、161 mM。钠和钾的这些米氏常数似乎与电压有关。3. 用两种不同的内部钠浓度以及外部为钠、内部为钾的情况测定了瞬时电流-电压关系。4. 这里表明，先前用于描述钠通道反转电位的三屏障、两位点离子渗透模型也能解释钠通道电流。
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