Prime S S, Toh B H
Cytobios. 1980;28(111-112):151-60.
Concanavalin A (Con A), insulin and epidermal growth factor (EGF) surface membrane receptors on cultured bovine odontogenic epithelial cells, were demonstrated by immunofluorescent reactivity with fluorescein-isothiocyanate (FITC) conjugated Con A, FITC-insulin, or EGF and anti-EGF, traced with FITC goat anti-rabbit globulin. Con A, insulin and EGF receptors co-capped with each other and with cytoplasmic actin; the latter was demonstrated by anti-actin antibody (AAA) traced with fluorescein or rhodamine-labelled goat anti-human globulin. Capping of receptors was promoted by prior treatment of cells with cytochalasin B or colchicine, but inhibited by the presence of the drugs throughout the experimental procedure. Binding of a ligand to its receptor promoted capping of an unrelated ligand-bound receptor, a phenomenon that was inhibited by cytochalasin B or colchicine. These results suggest that surface receptors for insulin, EGF and Con A interact with each other and with cytoplasmic actin.
通过用异硫氰酸荧光素（FITC）偶联的伴刀豆球蛋白A（Con A）、FITC - 胰岛素或表皮生长因子（EGF）以及抗EGF与用FITC标记的山羊抗兔球蛋白进行免疫荧光反应，证实了培养的牛牙源性上皮细胞上存在Con A、胰岛素和EGF表面膜受体。Con A、胰岛素和EGF受体彼此之间以及与细胞质肌动蛋白共同帽化；后者通过用荧光素或罗丹明标记的山羊抗人球蛋白追踪的抗肌动蛋白抗体（AAA）得以证实。用细胞松弛素B或秋水仙碱预先处理细胞可促进受体的帽化，但在整个实验过程中药物的存在会抑制这种帽化。一种配体与其受体的结合会促进不相关的配体结合受体的帽化，这种现象会被细胞松弛素B或秋水仙碱抑制。这些结果表明，胰岛素、EGF和Con A的表面受体彼此之间以及与细胞质肌动蛋白相互作用。
