Prime S S, Sim F R, Toh B H
J Pathol. 1983 Apr;139(4):431-40. doi: 10.1002/path.1711390404.
Human ameloblastoma tissue was investigated using cell culture techniques, transmission electron microscopy and fluorescent microscopy. Cultured cellular morphology was dependent on the type of substratum, with polygonal cells predominant on collagen substrata in contrast to an elongated cellular morphology on glass substrata. The presence of tonofilaments and desmosomes confirmed the epithelial origin of these cells. The distribution of Con A surface receptors and cytoplasmic actin in the same cell was studied using a double fluorochrome technique. Incubation with fluorescein isothiocyanate-labelled Con A at 37 degrees C for increasing time periods resulted in the Con A receptors showing progressive changes in staining patterns from clusters, to caps to perinuclear globules. Sequential changes in cytoplasmic actin, labelled by a specific anti-actin auto-antibody traced with rhodamine-labelled goat anti-human globulin, corresponded to the Con A staining patterns.
采用细胞培养技术、透射电子显微镜和荧光显微镜对人成釉细胞瘤组织进行了研究。培养的细胞形态取决于基质类型，与玻璃基质上细长的细胞形态相比，胶原基质上以多边形细胞为主。张力丝和桥粒的存在证实了这些细胞的上皮来源。使用双荧光染料技术研究了同一细胞中刀豆球蛋白A表面受体和细胞质肌动蛋白的分布。在37℃下用异硫氰酸荧光素标记的刀豆球蛋白A孵育不同时间，刀豆球蛋白A受体的染色模式从簇状逐渐变为帽状，再变为核周小球状。用罗丹明标记的山羊抗人球蛋白追踪的特异性抗肌动蛋白自身抗体标记的细胞质肌动蛋白的连续变化与刀豆球蛋白A染色模式相对应。
