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水泡性口炎病毒在小鼠脾细胞中的复制:病毒复制淋巴细胞的富集及复制限制分析

Replication of vesicular stomatitis virus in murine spleen cells: enrichment of the virus-replicating lymphocytes and analysis of replication restriction.

作者信息

Webb D R, Munshi S, Banerjee A K

出版信息

Infect Immun. 1981 Apr;32(1):169-72. doi: 10.1128/iai.32.1.169-172.1981.

Abstract

Vesicular stomatitis virus (Indiana strain) will only grow in T lymphocytes which have been stimulated to undergo cell division. Evidence is presented that a considerable enrichment of the vesicular stomatitis virus-replicating T cells may be accomplished in the mouse spleen by passing the spleen cells over glass wool columns. By using this procedure experiments were performed to study the nature of the block in vesicular stomatitis virus replication in unstimulated (nonpermissive) versus mitogen-stimulated (permissive) splenic T cells. The results show that, as is the case in permissive T-cell lines, stimulated normal T cells allow the synthesis of the 42S virion ribonucleic acid.

摘要

水泡性口炎病毒(印第安纳株)仅在被刺激进行细胞分裂的T淋巴细胞中生长。有证据表明,通过将脾细胞通过玻璃棉柱,可在小鼠脾脏中实现大量富集水泡性口炎病毒复制的T细胞。利用该程序进行了实验,以研究未刺激(非允许性)与丝裂原刺激(允许性)脾T细胞中水泡性口炎病毒复制的阻滞性质。结果表明,与允许性T细胞系的情况一样,受刺激的正常T细胞允许42S病毒粒子核糖核酸的合成。

相似文献

9
Studies on the interactions between viruses and lymphocytes.病毒与淋巴细胞之间相互作用的研究。
Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:73-83. doi: 10.1101/sqb.1977.041.01.011.

本文引用的文献

6
Studies on the interactions between viruses and lymphocytes.病毒与淋巴细胞之间相互作用的研究。
Cold Spring Harb Symp Quant Biol. 1977;41 Pt 1:73-83. doi: 10.1101/sqb.1977.041.01.011.

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