Webb D R, Munshi S, Banerjee A K
Infect Immun. 1981 Apr;32(1):169-72. doi: 10.1128/iai.32.1.169-172.1981.
Vesicular stomatitis virus (Indiana strain) will only grow in T lymphocytes which have been stimulated to undergo cell division. Evidence is presented that a considerable enrichment of the vesicular stomatitis virus-replicating T cells may be accomplished in the mouse spleen by passing the spleen cells over glass wool columns. By using this procedure experiments were performed to study the nature of the block in vesicular stomatitis virus replication in unstimulated (nonpermissive) versus mitogen-stimulated (permissive) splenic T cells. The results show that, as is the case in permissive T-cell lines, stimulated normal T cells allow the synthesis of the 42S virion ribonucleic acid.
水泡性口炎病毒(印第安纳株)仅在被刺激进行细胞分裂的T淋巴细胞中生长。有证据表明,通过将脾细胞通过玻璃棉柱,可在小鼠脾脏中实现大量富集水泡性口炎病毒复制的T细胞。利用该程序进行了实验,以研究未刺激(非允许性)与丝裂原刺激(允许性)脾T细胞中水泡性口炎病毒复制的阻滞性质。结果表明,与允许性T细胞系的情况一样,受刺激的正常T细胞允许42S病毒粒子核糖核酸的合成。