Stadecker M J
J Immunol. 1981 May;126(5):1724-7.
Thymidine was found in culture supernatants from both normal peritoneal macrophages and macrophages elicited in vivo with LPS under conditions in which there was no detectable TK activity. Abrogation of thymidine secretion was observed only when elicited macrophages were cultured in the presence of macrophage growth factor (MGF), whereas resident macrophages continued secreting under similar conditions. Similarly, TK activity was induced with MGF only in elicited, but not in resident, macrophages; LPS added in vitro failed to render resident macrophages susceptible to stimulation by MGF. Tests for adenosine and deoxyadenosine in macrophage supernatants proved that these nucleosides were not secreted by these cells. Although AK was consistently expressed in both resident and elicited macrophages, no significant dAK levels could be detected in either cell population. In contrast to TK, both purine nucleoside kinases could not be modulated or induced with MGF. The data indicate different reactivity to MGF by resident and elicited macrophages and a difference in pyrimidine vs purine nucleoside secretion as well as nucleoside kinase expression by these cells.
在无可检测到的胸苷激酶（TK）活性的条件下，在来自正常腹膜巨噬细胞以及经脂多糖（LPS）体内诱导的巨噬细胞的培养上清液中发现了胸苷。仅当诱导的巨噬细胞在巨噬细胞生长因子（MGF）存在的情况下培养时，才观察到胸苷分泌的消除，而驻留巨噬细胞在类似条件下继续分泌。同样，MGF仅在诱导的巨噬细胞中诱导TK活性，而在驻留巨噬细胞中则不诱导；体外添加的LPS未能使驻留巨噬细胞易于受到MGF的刺激。对巨噬细胞上清液中的腺苷和脱氧腺苷的检测证明这些核苷不是由这些细胞分泌的。尽管腺苷激酶（AK）在驻留和诱导的巨噬细胞中均持续表达，但在这两种细胞群体中均未检测到明显的脱氧腺苷激酶（dAK）水平。与TK不同，两种嘌呤核苷激酶均不能被MGF调节或诱导。数据表明驻留和诱导的巨噬细胞对MGF的反应性不同，以及这些细胞在嘧啶与嘌呤核苷分泌以及核苷激酶表达方面存在差异。
