Harris D T, Hale A H, Lefrancois L
J Immunol. 1981 May;126(5):1914-8.
We have analyzed the requirement for the expression of the major surface glycoprotein (G protein) of vesicular stomatitis virus (VSV) on target cells for recognition and lysis by anti-VSV cytotoxic T lymphocytes (CTL). In addition, we have attempted to determine if the carbohydrate moieties on the G protein are required for recognition and lysis by anti-VSV CTL. When VSV (Orsay) is grown at 30 degrees C in the presence of tunicamycin (TM), glycosylation of G protein is inhibited; however, nonglycosylated G protein is found on the surface of the cell and active virus particles are produced. In contrast, VSV (Orsay) grown at 39 degrees C in the presence of TM produces low titers of virus and the presence of G protein on the surface of cells is not detectable. The susceptibility of these target cells to lysis by anti-VSV CTL was analyzed. The results suggest that expression of the G protein is required for target cell lysis by anti-VSV CTL. However, the presence of the carbohydrate moieties on the G protein are nt an absolute requirement for recognition by anti-VSV CTL. VSV-infected target cells incubated in the presence of TM were lysed by anti-VSV CTL up to 50 to 80% of the infected target cell control. This result suggests either that some clones of anti-VSV CTL recognize carbohydrate moieties or that carbohydrate moieties play some as yet undefined nonantigenic role in the recognition of the target antigen by the CTL receptor.
我们分析了水泡性口炎病毒（VSV）主要表面糖蛋白（G蛋白）在靶细胞上表达对于抗VSV细胞毒性T淋巴细胞（CTL）识别和裂解的必要性。此外，我们试图确定G蛋白上的碳水化合物部分对于抗VSV CTL的识别和裂解是否必要。当VSV（奥赛株）在30摄氏度下于衣霉素（TM）存在的情况下生长时，G蛋白的糖基化受到抑制；然而，未糖基化的G蛋白可在细胞表面发现，并且能产生有活性的病毒颗粒。相比之下，VSV（奥赛株）在39摄氏度下于TM存在的情况下生长产生的病毒滴度较低，并且在细胞表面无法检测到G蛋白的存在。分析了这些靶细胞对抗VSV CTL裂解的敏感性。结果表明，G蛋白的表达是抗VSV CTL裂解靶细胞所必需的。然而，G蛋白上碳水化合物部分的存在并非抗VSV CTL识别的绝对必要条件。在TM存在的情况下孵育的VSV感染靶细胞被抗VSV CTL裂解，可达感染靶细胞对照的50%至80%。这一结果表明，要么某些抗VSV CTL克隆识别碳水化合物部分，要么碳水化合物部分在CTL受体识别靶抗原过程中发挥某种尚未明确的非抗原作用。
