Tachibana S, Oka M
J Nutr Sci Vitaminol (Tokyo). 1980;26(5):419-26. doi: 10.3177/jnsv.26.419.
A method for enzymatic determination of riboflavin was developed by enzymatic reduction of 2,6-dichlorophenolindophenol using a vitamin B2-aldehyde-forming enzyme prepared from Schizophyllum commune. The enzymatic method enabled us to determine riboflavin in a mixture containing riboflavin, FMN and FAD without separation of riboflavin. Moreover, the total amount of flavins in the mixture was shown to be determined by the enzyme in the presence of phosphatase.
利用从裂褶菌制备的维生素B2醛形成酶，通过酶促还原2,6-二氯酚靛酚，开发了一种测定核黄素的方法。该酶法使我们能够在不分离核黄素的情况下，测定含有核黄素、黄素单核苷酸（FMN）和黄素腺嘌呤二核苷酸（FAD）的混合物中的核黄素。此外，在磷酸酶存在的情况下，混合物中黄素的总量可由该酶测定。
