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[鳗鱼中α-促黑素细胞激素(α-MSH)和促肾上腺皮质激素(ACTH)在铅苏木精(HPb)可染色垂体细胞中的细胞免疫定位]

[Cyto-immunologic location of alpha-MSH and ACTH in the lead-hematoxylin (HPb)-stainable pituitary cells, in the eel].

作者信息

Olivereau M, Bugnon C, Fellmann D

出版信息

C R Acad Hebd Seances Acad Sci D. 1976 Nov 8;283(11 D):1321-3.

PMID:62625
Abstract

The lead-haematoxylin positive cells of the pars intermedia react with anti-alpha-MSH and anti-1-24ACTH or anti-17-39ATCH; those of the rostal pars distalis are only revealed with antisera anti-1-24ACTH and anti-17-39ACTH. Intensity of cytoimmunological staining, which is modified after various experimental treatments (reserpine, metyrapon, pimozid, cortisol...) and during black or white background adaptaiton, corresponds essentially to that of the PbH staining.

摘要

中间部的铅苏木精阳性细胞与抗α-促黑素、抗1-24促肾上腺皮质激素或抗17-39促肾上腺皮质激素发生反应;远侧部头端的细胞仅用抗1-24促肾上腺皮质激素和抗17-39促肾上腺皮质激素抗血清才能显示。细胞免疫染色的强度在各种实验处理(利血平、甲吡酮、匹莫齐特、皮质醇……)后以及在黑白背景适应过程中会发生改变,其强度基本上与铅苏木精染色的强度一致。

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