English D, Roloff J S, Lukens J N
Blood. 1981 Jul;58(1):129-34.
Human neutrophils exposed to chemotactic concentrations of zymosan-activated serum (ZAS) and a formylated chemotactic peptide (FMLP, 10(-7)--10(-9) M) were markedly enhanced in their ability to generate superoxide (O2-) upon stimulation with either sodium fluoride or phorbol myristate acetate (PMA). For both fluoride and PMA, enhancement was characterized by a decrease in the lag from stimulation to initiation of superoxide release and by an increase in the rate of superoxide generation--representing faster activation and increased activity of O2- generating enzyme, respectively. Chemotactic concentrations of casein, normal serum, and casein-treated serum enhanced the activity, but not the rate of activation, of the fluoride-stimulated superoxide generating system. This effect on activity was not so impressive as that obtained with FMLP or ZAS. The mechanisms by which FMLP enhanced responsiveness to fluoride and PMA were found to be different. Optimal enhancement for fluoride-stimulated responses required extracellular Ca++. Extracellular glucose, but not extracellular Ca++, was required for enhancement of FMLP of PMA-stimulated responses. A similar glucose requirement could not be demonstrated for chemotactic peptide enhancement of the superoxide-generating system stimulated by fluoride. Fluoride and PMA apparently activate the neutrophil O2- generating enzyme by pathways that are not identical. However, responsiveness of the enzyme to both agents is susceptible to modulation by cellular responses to chemotactic peptides.
暴露于趋化浓度的酵母聚糖激活血清（ZAS）和一种甲酰化趋化肽（FMLP，10⁻⁷ - 10⁻⁹ M）的人中性粒细胞，在用氟化钠或佛波酯肉豆蔻酸酯（PMA）刺激时，其产生超氧化物（O₂⁻）的能力显著增强。对于氟化钠和PMA两者而言，增强作用的特征在于从刺激到超氧化物释放开始的延迟时间缩短，以及超氧化物生成速率增加，分别代表O₂⁻生成酶的更快激活和活性增加。趋化浓度的酪蛋白、正常血清和酪蛋白处理的血清增强了氟化钠刺激的超氧化物生成系统的活性，但没有增强其激活速率。这种对活性的影响不如用FMLP或ZAS获得的影响那么显著。发现FMLP增强对氟化钠和PMA反应性的机制是不同的。氟化钠刺激反应的最佳增强需要细胞外Ca²⁺。PMA刺激反应的FMLP增强需要细胞外葡萄糖，但不需要细胞外Ca²⁺。对于氟化钠刺激的超氧化物生成系统的趋化肽增强，未证明有类似的葡萄糖需求。氟化钠和PMA显然通过不同的途径激活中性粒细胞的O₂⁻生成酶。然而，该酶对这两种试剂的反应性易受细胞对趋化肽反应的调节。
