Reitherman R, Chen L, Wen S, Harding B W
Endocrinology. 1981 Jul;109(1):301-6. doi: 10.1210/endo-109-1-301.
A cAMP-binding protein and cAMP-dependent protein kinase have been identified and partially characterized in bovine adrenocortical plasma membranes. [3H]cAMP binding to the plasma membrane preparation demonstrates both high (Ka = 4.2 X 10(9) M-1) and low (Ka = 1.3 X 10(8) M-1) affinity binding sites. cAMP-dependent protein kinase was demonstrated using both bovine histone and endogenous substrates. The detergent-dependent solubilization characteristics of this cAMP-dependent protein kinase indicate that it is an integral membrane protein.
