在几种人类肿瘤细胞系中检测到一种共同特征——一种53,000道尔顿的蛋白质。

Crawford L V, Pim D C, Gurney E G, Goodfellow P, Taylor-Papadimitriou J

Proc Natl Acad Sci U S A. 1981 Jan;78(1):41-5. doi: 10.1073/pnas.78.1.41.

Human cell lines, whether derived from spontaneous tumors or transformed in vitro with simian virus 40, were found to contain a 53,000-dalton phosphoprotein (pp53) in contrast to normal human cells in which this protein was not detected. Isoelectric focusing showed that pp53 comprised several species in both simian virus 40-transformed and tumor cells. Comparison of the pp53 species from the various cell lines by partial proteolysis showed that they were similar but not identical. Among the 13 tumor cell lines examined, only 1 line, HeLa, did not contain detectable pp53. All the other tumor cell lines contained pp53, and it is suggested that this protein may be associated with their transformed state.

人们发现，无论是源自自发肿瘤还是用猿猴病毒40在体外转化得到的人类细胞系，都含有一种53,000道尔顿的磷蛋白（pp53），而在正常人类细胞中未检测到这种蛋白质。等电聚焦显示，在猿猴病毒40转化的细胞和肿瘤细胞中，pp53都由几种不同的蛋白组成。通过部分蛋白酶解对来自不同细胞系的pp53进行比较，结果表明它们相似但并不完全相同。在所检测的13种肿瘤细胞系中，只有一种细胞系（HeLa）未检测到可检测到的pp53。所有其他肿瘤细胞系都含有pp53，有人认为这种蛋白质可能与它们的转化状态有关。