[体外从肾皮质合成环磷酸腺苷（作者译）]
[Synthesis of cyclic AMP from renal cortex in vitro (author's transl)].
作者信息
Camps J, Jiménez W, Llorens A, Rivera-Fillat F
出版信息
Rev Esp Fisiol. 1981 Mar;37(1):31-6.
The parathyroid-hormone sensitive adenylate-cyclase from renal rat cortex has been studied. Mg2+ acts as stimulator at a specific locus increasing the apparent Vmax slightly from 50 to 60 nmol of cAMP per gram of protein if the Mg2+ concentration increases from 3.25 to 16 mM, and decreases the apparent KM from 2 to 0.4 mM. Fluoride decreases the apparent KM from 5 mM in NaF concentrations of 0 to 2 mM in NaF concentrations 2 mM. Magnesium ion also decreases the apparent Ka for NaF.
对来自大鼠肾皮质的甲状旁腺激素敏感腺苷酸环化酶进行了研究。镁离子在特定位点起刺激作用，当镁离子浓度从3.25毫摩尔增加到16毫摩尔时，每克蛋白质产生的环磷酸腺苷（cAMP）表观最大反应速度（Vmax）略有增加，从50纳摩尔增加到60纳摩尔，并使表观米氏常数（KM）从2毫摩尔降至0.4毫摩尔。氟化物在氟化钠浓度为0至2毫摩尔时，可使表观KM从5毫摩尔降低至2毫摩尔。镁离子也会降低氟化物的表观解离常数（Ka）。
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