Rivedal E, Sanner T
Cancer Res. 1981 Jul;41(7):2950-3.
The hamster embryo cell bioassay has been used to study the effect of metal salts on morphological transformation. A synergistic enhancement of the transformation frequency was found for the combined treatment with organic carcinogens [benzo(a)pyrene, N-hydroxy-2-acetylaminofluorene, and 4-nitroquinoline 1-oxide] and nickel sulfate, cadmium acetate, or potassium chromate. Chromic chloride and zinc chloride did not induce transformation themselves, and they had no effect on the transformation frequency when tested in combination with benzo(a)pyrene. The synergistic effect between benzo(a)pyrene and nickel sulfate or cadmium acetate was also apparent when the cells were treated sequentially with the chemicals. When the cells were first exposed to benzo(a)pyrene, both nickel sulfate and cadmium acetate showed a promotion-like effect similar to that obtained with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. Moreover, when 12-O-tetradecanoylphorbol-13-acetate or benzo(a)pyrene were used as promoting agents, both nickel sulfate and cadmium acetate were able to initiate morphological transformation. The data suggest that the metal salts are more potent as promoters than they are as initiators. The present findings may be of importance in relation to carcinogenicity of metal compounds to humans.
仓鼠胚胎细胞生物测定法已被用于研究金属盐对形态转化的影响。对于有机致癌物[苯并(a)芘、N-羟基-2-乙酰氨基芴和4-硝基喹啉1-氧化物]与硫酸镍、醋酸镉或铬酸钾的联合处理，发现转化频率有协同增强作用。氯化铬和氯化锌本身不诱导转化，并且当与苯并(a)芘联合测试时，它们对转化频率没有影响。当细胞依次用这些化学物质处理时，苯并(a)芘与硫酸镍或醋酸镉之间的协同效应也很明显。当细胞首先暴露于苯并(a)芘时，硫酸镍和醋酸镉都显示出类似于用肿瘤促进剂12-O-十四烷酰佛波醇-13-乙酸酯所获得的促进样效应。此外，当使用12-O-十四烷酰佛波醇-13-乙酸酯或苯并(a)芘作为促进剂时，硫酸镍和醋酸镉都能够引发形态转化。数据表明，金属盐作为促进剂比作为引发剂更有效。目前的发现可能与金属化合物对人类的致癌性有关。
