人肝癌细胞系中表皮生长因子对鸟氨酸脱羧酶活性的刺激作用。
Epidermal growth factor stimulation of ornithine decarboxylase activity in a human hepatoma cell line.
作者信息
Moriarity D M, DiSorbo D M, Litwack G, Savage C R
出版信息
Proc Natl Acad Sci U S A. 1981 May;78(5):2752-6. doi: 10.1073/pnas.78.5.2752.
Epidermal growth factor (EGF) bound specifically to the human hepatoma cell line PLC/PRF/5. Treatment of these cells with nanomolar concentrations of EGF for 4-6 hr resulted in a 2- to 6-fold increase in ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activity. 12-O-Tetradecanoylphorbol 13-acetate also produced an increase in enzyme activity in these cells and exhibited an additive effect with EGF. It did not inhibit the binding of 125I-labeled EGF to these cells. The stimulation of enzyme activity by EGF was not inhibited by cycloheximide or actinomycin D, although these agents did cause a significant decrease in enzyme levels when added without EGF. Also, colchicine, chloroquine, ammonium chloride, and methylamine, compounds that inhibit EGF degradation in various cells types, did not interfere with the ability of EGF to elevate enzyme levels in the human hepatoma cells.
表皮生长因子（EGF）能特异性地结合人肝癌细胞系PLC/PRF/5。用纳摩尔浓度的EGF处理这些细胞4至6小时，可使鸟氨酸脱羧酶（L-鸟氨酸羧基裂解酶，EC 4.1.1.17）的活性增加2至6倍。12-O-十四酰佛波醇-13-乙酸酯也能使这些细胞中的酶活性增加，并与EGF表现出相加效应。它不抑制125I标记的EGF与这些细胞的结合。尽管在无EGF的情况下添加放线菌酮或放线菌素D会导致酶水平显著降低，但它们并不抑制EGF对酶活性的刺激作用。此外，秋水仙碱、氯喹、氯化铵和甲胺等能抑制不同细胞类型中EGF降解的化合物，并不干扰EGF提高人肝癌细胞中酶水平的能力。
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