Conformational changes of yeast tRNAPhe upon interaction with intercalators probed by nuclease digestion.

The conformational changes of yeast-tRNAPhe induced by various intercalators have been studied using limited digestions with nucleases S1 and T1. The results show an increased sensitivity to T1 in the D-loop, suggesting a weakening of the D-loop-T-loop interaction. Furthermore, the results are best explained by a non-intercalative binding of the eyes, probably in the D-loop-T-loop cavity.