Schotman P, Allaart J, Gispen W H
Brain Res. 1981 Aug 24;219(1):121-35. doi: 10.1016/0006-8993(81)90272-9.
Pineal protein synthesis was studied in vitro over a period of 6-8 h after dissection. The level of protein synthetic activity of the pineal gland was greatly dependent on the time of dissection showing a maximum at midnight and a minimum at 10.00 h, 2 h after onset of light. Low concentrations of ACTH1-24 (down to 10(-11) M) could stimulate protein synthesis in vitro. The sensitivity to hormonal stimulation showed a circadian variation similar to that observed in the basal protein synthetic activity. Furthermore, overall synthetic activity appeared to be under neural influence. These neural and hormonal influences seemed to be mediated by beta-receptor stimulation and cyclic AMP. Structure-activity studies of the ACTH-effect on pineal protein synthesis gave results similar to those previously observed for excessive grooming behaviour, synaptic plasma membrane phosphorylation, adenylcyclase-activity and cell-free protein synthesis in brain. It was concluded, that overall pineal protein synthesis is both under neural and hormonal control. The action of ACTH on protein synthesis rate might be mediated by a calcium-dependent release of norepinephrine followed postsynaptically by beta-receptor activation, cAMP production, and stimulation of translation.
在解剖后的6 - 8小时内，对松果体蛋白质合成进行了体外研究。松果体蛋白质合成活性水平在很大程度上取决于解剖时间，在午夜达到最高，在光照开始后2小时即10:00时达到最低。低浓度的促肾上腺皮质激素1 - 24（低至10^(-11) M）可在体外刺激蛋白质合成。对激素刺激的敏感性表现出与基础蛋白质合成活性中观察到的类似的昼夜节律变化。此外，总体合成活性似乎受神经影响。这些神经和激素影响似乎是由β受体刺激和环磷酸腺苷介导的。促肾上腺皮质激素对松果体蛋白质合成影响的构效关系研究结果与先前在过度梳理行为、突触质膜磷酸化、腺苷酸环化酶活性和脑内无细胞蛋白质合成中观察到的结果相似。得出的结论是，松果体总体蛋白质合成受神经和激素的双重控制。促肾上腺皮质激素对蛋白质合成速率的作用可能是由去甲肾上腺素的钙依赖性释放介导的，随后在突触后发生β受体激活、环磷酸腺苷生成和翻译刺激。
