Inoue T, Carsten A L, Cronkite E P, Kelley J E
Exp Hematol. 1981 Jul;9(6):563-72.
To obtain concentrated suspensions of pluripotent hematopoietic stem cells *CFUS) from murine bone marrow, density gradient centrifugal sedimentation (DGCS) was combined with counterflow centrifugal elutriation (CE). This combination provided a 7.6 fold enrichment of the CFUS concentration. For DGCS, Percoll a suspension of silica particles coated with polyvinylpyrrolidone was used. For fractionation by the CE an elutriator rotor (JE-6, Beckman) was used for further concentration of the cells harvested from the DGCS. Bone marrow erythropoiesis was suppressed by transfusion plethora initiated 5-6 days before the bone marrow was harvested. These two physical separation procedures combined with transfusion plethora to suppress erythropoiesis are effective in producing an enriched fraction of CFUS without change in distribution of the histologic type of colonies.
为了从小鼠骨髓中获得多能造血干细胞集落形成单位（CFUS）的浓缩悬液，将密度梯度离心沉降（DGCS）与逆流离心淘洗（CE）相结合。这种组合使CFUS浓度提高了7.6倍。对于DGCS，使用了包被有聚乙烯吡咯烷酮的二氧化硅颗粒悬浮液Percoll。为了通过CE进行分级分离，使用淘洗转子（JE-6，贝克曼）对从DGCS收获的细胞进行进一步浓缩。在收获骨髓前5 - 6天通过输血过多抑制骨髓红细胞生成。这两种物理分离程序与输血过多抑制红细胞生成相结合，可有效产生CFUS富集部分，而不改变集落组织学类型的分布。
