Baba M L, Darga L L, Goodman M, Czelusniak J
J Mol Evol. 1981;17(4):197-213. doi: 10.1007/BF01732758.
Rates of evolution for cytochrome c over the past one billion years were calculated from a maximum parsimony dendrogram which approximates the phylogeny of 87 lineages. Two periods of evolutionary acceleration and deceleration apparently occurred for the cytochrome c molecule. The tempo of evolutionary change indicated by this analysis was compared to the patterns of acceleration and deceleration in the ancestry of several other proteins. The synchrony of these tempos of molecular change supports the notion that rapid genetic evolution accompanied periods of major adaptive radiations. Rates of change at different time in several structural-functional areas of cytochrome c were also investigated in order to test the Darwinian hypothesis that during periods of rapid evolution, functional sites accumulate proportionately more substitutions than areas with no known functions. Rates of change in four proposed functional groupings of sites were therefore compared to rates in areas of unknown function for several different time periods. This analysis revealed a significant increase in the rate of evolution for sites associated with the regions of cytochrome c oxidase and reductase interaction during the period between the emergence of the eutherian ancestor to the emergence of the anthropoid ancestor.
通过一个近似87个谱系系统发育的最大简约树状图，计算了过去十亿年细胞色素c的进化速率。细胞色素c分子显然经历了两个进化加速和减速阶段。该分析所显示的进化变化速度与其他几种蛋白质谱系中的加速和减速模式进行了比较。这些分子变化速度的同步性支持了这样一种观点，即快速的基因进化伴随着主要适应性辐射期。为了检验达尔文的假说，即在快速进化时期，功能位点比无已知功能的区域积累的替换比例更高，还研究了细胞色素c几个结构功能区域在不同时间的变化速率。因此，在几个不同的时间段内，将四个提议的位点功能分组的变化速率与未知功能区域的变化速率进行了比较。该分析揭示，在真兽类祖先出现到类人猿祖先出现的时期，与细胞色素c氧化酶和还原酶相互作用区域相关的位点进化速率显著增加。
