来自BRL 3A大鼠肝细胞系的增殖刺激活性（MSA）：与人生长调节素和胰岛素的关系。

Multiplication stimulating activity (MSA) from the BRL 3A rat liver cell line: relation to human somatomedins and insulin.

作者信息

Rechler M M, Nissley S P, King G L, Moses A C, Van Obberghen-Schilling E E, Romanus J A, Knight A B, Short P A, White R M

出版信息

J Supramol Struct Cell Biochem. 1981;15(3):253-86. doi: 10.1002/jsscb.1981.380150305.

DOI:10.1002/jsscb.1981.380150305

PMID:6267316

Abstract

The properties of multiplication stimulating activity (MSA), an insulin-like growth factor (somatomedin) purified from culture medium conditioned by the BRL 3A rat liver cell line are summarized. The relationship of MSA to somatomedins purified from human and rat plasma are considered. MSA appears to be the predominant somatomedin in fetal rat serum, but a minor component of adult rat somatomedin. In vitro biological effects of MSA and insulin in adipocytes, fibroblasts and chondrocytes are examined to determine whether they are mediated by insulin receptors or insulin-like growth factor receptors. The possible relationship of a primary defect of insulin receptors observed in fibroblasts from a patient with the rare genetic disorder, leprechaunism, to intrauterine growth retardation is discussed.

摘要

总结了从BRL 3A大鼠肝细胞系条件培养基中纯化得到的一种胰岛素样生长因子（生长调节素）——增殖刺激活性（MSA）的特性。探讨了MSA与从人和大鼠血浆中纯化得到的生长调节素之间的关系。MSA似乎是胎鼠血清中主要的生长调节素，但在成年大鼠生长调节素中是次要成分。研究了MSA和胰岛素在脂肪细胞、成纤维细胞和软骨细胞中的体外生物学效应，以确定它们是由胰岛素受体还是胰岛素样生长因子受体介导的。讨论了在患有罕见遗传病“妖精貌综合征”患者的成纤维细胞中观察到的胰岛素受体原发性缺陷与宫内生长迟缓之间的可能关系。