Katsuta K, Tamai Y, Watanabe T, Fujita S, Satake M
Biochim Biophys Acta. 1978 Feb 21;507(2):271-9. doi: 10.1016/0005-2736(78)90422-4.
The light smooth-surfaced membrane, one of the three membrane fractions derived from the rat brain microsomal fraction, was fractionated into its soluble and insoluble parts by the use of lysophosphatidylcholine and the chemical composition of these was investigated. Under the condition whereby the maximal amount of the membrane protein was solubilized by lysophosphatidylcholine (0.5% lysophosphatidylcholine at 37 degrees C for 10 min), the insoluble residue, which accounted for approximately 30% of the membrane protein, was ultracentrifugally homogeneous and showed a granular structure under the electron microscope. The lipid composition of the soluble and insoluble fractions, as well as their protein composition, revealed a preferential and limited solubilization of the constituents of the membrane by lysophosphatidylcholine.
轻的光滑表面膜是从大鼠脑微粒体部分分离得到的三种膜部分之一，通过使用溶血磷脂酰胆碱将其分离为可溶部分和不可溶部分，并对其化学组成进行了研究。在溶血磷脂酰胆碱使膜蛋白溶解量达到最大的条件下（37℃下0.5%溶血磷脂酰胆碱作用10分钟），占膜蛋白约30%的不溶残余物经超速离心后是均一的，在电子显微镜下呈颗粒状结构。可溶部分和不可溶部分的脂质组成及其蛋白质组成表明，溶血磷脂酰胆碱对膜成分有优先且有限的溶解作用。
