Khlystola Z S, Zaĭtsev T I
Biull Eksp Biol Med. 1978 Jan;85(1):81-4.
The liver and spleen of gnotobiotic Wistar rats were examined by histochemical methods; the liver was also studied by electron microscopy. Under conditions of gnotobiotic animal existance the activity of succinic dehydrogenase, non-specific esterase in the liver was found to be decreased; fatty infiltration was intensified in the cytoplasm of hepatocytes and Kupffer's cells; a part of them is subject to fatty dystophy. At the same time both the activity of acid phosphatase of hepatocytes and the contents of lysosomes in the biliary poles of hepatocytes became increased. Decomposition of erythrocytes in the spleen, as well as release of free iron and pigments which stimulated excretion of bile in gnotobiotes were enhanced.
用组织化学方法检查了无菌Wistar大鼠的肝脏和脾脏；还用电子显微镜对肝脏进行了研究。在无菌动物生存条件下，发现肝脏中琥珀酸脱氢酶、非特异性酯酶的活性降低；肝细胞和库普弗细胞的细胞质中脂肪浸润加剧，部分细胞发生脂肪营养不良。同时，肝细胞酸性磷酸酶的活性以及肝细胞胆小管处溶酶体的含量均增加。无菌动物脾脏中红细胞的分解以及游离铁和色素的释放增强，这些物质刺激了胆汁的排泄。
