Brody A R, Hill L H, Adkins B, O'Connor R W
Am Rev Respir Dis. 1981 Jun;123(6):670-9. doi: 10.1164/arrd.1981.123.6.670.
The initial deposition and subsequent translocation of chrysotile asbestos were studied in the lungs of rats exposed for 1 h in inhalation chambers. Using scanning and transmission electron microscopy of tissue fixed by vascular perfusion, we determined that the majority of fibers that pass through the conducting airways deposits at the bifurcations of alveolar ducts. The farther an alveolar duct bifurcation was from its terminal bronchiole, the less asbestos were observed. The amount of asbestos present on the alveolar duct surfaces was significantly decreased 5 h after cessation of the 1-h exposure. Some fibers were taken up by Type I epithelial cells during the first hour of dusting, and this process continued through the 8-day period in which the animals were studied. As early as 24 h after exposure, there was an accumulation of macrophages at the sites of initial asbestos deposition. This may be a significant cellular response in the early pathogenesis of asbestosis.
在吸入舱中暴露1小时的大鼠肺部，研究了温石棉的初始沉积及随后的迁移情况。通过对经血管灌注固定的组织进行扫描和透射电子显微镜检查，我们确定，穿过传导气道的大多数纤维沉积在肺泡管的分叉处。肺泡管分叉处离终末细支气管越远，观察到的石棉越少。在1小时暴露停止后5小时，肺泡管表面存在的石棉量显著减少。在撒尘后的第一小时，一些纤维被I型上皮细胞摄取，并且在研究动物的8天期间这个过程持续存在。早在暴露后24小时，在最初石棉沉积部位就有巨噬细胞聚集。这可能是石棉沉着病早期发病机制中的一种重要细胞反应。
