叙利亚仓鼠胚胎细胞对苯并[a]芘和苯并[e]芘的体外摄取与释放随血清浓度的变化情况。

The uptake and release of benzo[a]pyrene and benzo[e]pyrene in vitro by Syrian hamster embryo cells as a function of serum concentration.

作者信息

Coulomb H, Gu Z, Audu S, Chouroulinkov I

出版信息

Carcinogenesis. 1981;2(6):523-7. doi: 10.1093/carcin/2.6.523.

DOI:10.1093/carcin/2.6.523

Abstract

A quantitative study on the in vitro uptake of benzo[a]pyrene (B[a]P) and benzo[e]pyrene (B[e]P) by Syrian hamster embryo cells and the induction of sister chromatid exchange (SCE) has been carried out. The amounts of B[a]P and B[e]P taken up by the cells decreases as does the induction of SCEs by B[a]P when the concentration of serum in the culture medium increases. It appears that serum prevents (B[a]P or B[e]P uptake. We have observed no significant differences between the two hydrocarbons regarding uptake by cells; chromatographic results show however that B[a]P is metabolized by these cells, while B[e]P is not. Our results suggest that serum inhibits B[a]P and B[e]P uptake and hence decreases the number of SCEs.

摘要

已对叙利亚仓鼠胚胎细胞对苯并[a]芘（B[a]P）和苯并[e]芘（B[e]P）的体外摄取以及姐妹染色单体交换（SCE）的诱导进行了定量研究。当培养基中血清浓度增加时，细胞摄取的B[a]P和B[e]P的量以及B[a]P诱导的SCE均减少。似乎血清会阻止（B[a]P或B[e]P的摄取）。我们观察到这两种碳氢化合物在细胞摄取方面没有显著差异；然而，色谱结果表明，这些细胞会代谢B[a]P，而不会代谢B[e]P。我们的结果表明，血清会抑制B[a]P和B[e]P的摄取，从而减少SCE的数量。