Dionne V E
Fed Proc. 1981 Sep;40(11):2614-7.
Acetylcholine receptors in slow fiber neuromuscular junctions of garter snake (sp. Thamnophis) produced synaptic responses that were more complicated than those observed from twitch fibers. Although the slow fiber miniature end plate currents decayed monoexponentially with time, both the current fluctuations spectrum and the voltage jump end plate current required two temporal components for good theoretical fits. This behavior was accurately accounted for by a generalized version of the three-state kinetic model by del Castillo and Katz. Application of the model allowed not only the rate of channel closing to be estimated, but also the rate of channel opening (from the closed state with acetylcholine bound) and the apparent rate of acetylcholine unbinding from the receptor. The results suggest that at the peak of the miniature end plate current local receptor saturation occurs.
束带蛇（学名：锦蛇属）慢肌纤维神经肌肉接头处的乙酰胆碱受体产生的突触反应比在快肌纤维中观察到的更为复杂。尽管慢肌纤维微小终板电流随时间呈单指数衰减，但电流波动频谱和电压阶跃终板电流都需要两个时间成分才能得到良好的理论拟合。这种行为可以通过德尔卡斯蒂略和卡茨三态动力学模型的广义版本得到准确解释。该模型的应用不仅可以估计通道关闭的速率，还可以估计通道开放的速率（从乙酰胆碱结合的关闭状态）以及乙酰胆碱从受体上解离的表观速率。结果表明，在微小终板电流峰值时会发生局部受体饱和。
