Kawakami K, Ishihama A, Hamaguchi M
J Biochem. 1981 Jun;89(6):1751-7. doi: 10.1093/oxfordjournals.jbchem.a133374.
A systematic and comparative study was performed on the polypeptide composition and the RNA polymerase activity associated with virions of various strains of influenza A virus, including four human and two avian viruses. Significant differences were found in the molecular weights of not only hemagglutinin (HA) but also both nucleoprotein (NP) and membrane protein (M), as determined by polyacrylamide gel electrophoresis under denaturing conditions. The results indicate that, among viruses sharing the same serotype determined by the surface proteins HA and NA (neuraminidase), considerable variations exist in the structure of viral proteins, including inner proteins. The relative contents of viral proteins also varied among these strains grown under similar conditions. The total content of three P proteins, the putative RNA polymerase subunits, was within the range between 1.1 and 2.2% of total viral proteins and roughly paralleled the virion-associated RNA polymerase activity. The virion-associated RNA polymerase of all the strains tested were stimulated by the same dinucleotide primers, ApG or GpG, indicating that the specificity of transcription initiation is conserved among wide varieties of influenza virus.
对包括四种人类病毒和两种禽病毒在内的甲型流感病毒各毒株的病毒粒子相关的多肽组成和RNA聚合酶活性进行了系统的比较研究。在变性条件下通过聚丙烯酰胺凝胶电泳测定发现，不仅血凝素（HA），而且核蛋白（NP）和膜蛋白（M）的分子量都存在显著差异。结果表明，在由表面蛋白HA和神经氨酸酶（NA）确定的相同血清型的病毒中，包括内部蛋白在内的病毒蛋白结构存在相当大的差异。在相似条件下培养的这些毒株中，病毒蛋白的相对含量也有所不同。三种假定的RNA聚合酶亚基P蛋白的总含量在病毒总蛋白的1.1%至2.2%之间，并且大致与病毒粒子相关的RNA聚合酶活性平行。所有测试毒株的病毒粒子相关RNA聚合酶都受到相同的二核苷酸引物ApG或GpG的刺激，这表明转录起始的特异性在多种流感病毒中是保守的。
