Robinson J B, Brems D N, Stellwagen E
J Biol Chem. 1981 Nov 10;256(21):10769-73.
Nucleoside diphosphate kinase (EC 2.7.4.6) has been purified nearly 2000-fold from beef brain particulate material in 65% yield. The purification procedure described here takes advantage of the relatively unique complexation of the enzyme with blue dextran-Sepharose in the presence of a nonionic detergent. Unlike preparations of the enzyme previously described, the bovine brain enzyme is monoisozymic and each polypeptide chain can be phosphorylated by a nucleoside triphosphate substrate. Catalytically functional forms of the enzyme having lower pI values can be generated by proteolysis.
核苷二磷酸激酶（EC 2.7.4.6）已从牛脑颗粒物质中纯化出来，纯化倍数接近2000倍，产率为65%。此处描述的纯化方法利用了该酶在非离子洗涤剂存在下与蓝色葡聚糖-琼脂糖的相对独特的络合作用。与先前描述的该酶制剂不同，牛脑酶是单同工酶，并且每条多肽链都可以被核苷三磷酸底物磷酸化。通过蛋白水解可以产生具有较低pI值的该酶的催化功能形式。
