Gutweniger H, Bisson R, Montecucco C
J Biol Chem. 1981 Nov 10;256(21):11132-6.
The membrane topology of ubiquinone-cytochrome c reductase (EC 1.10.2.2.) has been investigated with photoreactive lipid analogs (Bisson, R., and Montecucco, C. (1981) Biochem. J. 193, 757-763), both in its isolated form and when part of succinate-cytochrome c reductase (Complex II + III). These probes react specifically with those polypeptide chains exposed to lipids, thereby labeling them radioactively. Highly resolving gel electrophoretic conditions have been used to determine the patterns of labeling. Core protein I, cytochrome b, cytochrome c1, and polypeptides VI, VII, VIII, and IX contribute to the lipid-protein boundary of Complex III. Evidence that the interaction between Complex II and Complex III involves their hydrophobic domains is also presented.
已使用光反应性脂质类似物（比松，R.，和蒙特库科，C.（1981年）《生物化学杂志》193卷，757 - 763页）研究了泛醌 - 细胞色素c还原酶（EC 1.10.2.2.）的膜拓扑结构，研究对象既包括其分离形式，也包括其作为琥珀酸 - 细胞色素c还原酶（复合体II + III）一部分时的情况。这些探针与暴露于脂质的那些多肽链特异性反应，从而对它们进行放射性标记。已使用高分辨率凝胶电泳条件来确定标记模式。核心蛋白I、细胞色素b、细胞色素c1以及多肽VI、VII、VIII和IX构成了复合体III的脂质 - 蛋白质边界。还提供了证据表明复合体II和复合体III之间的相互作用涉及其疏水结构域。
