Schmitz H
J Immunol Methods. 1981;42(3):337-42. doi: 10.1016/0022-1759(81)90162-9.
An improved fixation method for antigen detection in lymphoblastoid cells is described. Herpesvirus papio nuclear antigen (HUPNA) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (ACIF). Antibody to HUPNA was detected in many human sera containing antibodies to Epstein-Barr virus capsid and nuclear antigen (EBNA). Rheumatoid arthritis sera showed a high incidence of both anti-EBNA and anti-HUPNA antibodies.
本文描述了一种用于检测淋巴母细胞中抗原的改良固定方法。通过抗补体免疫荧光法(ACIF),可在几种转化的淋巴细胞系中对猴疱疹病毒核抗原(HUPNA)进行染色。在许多含有抗爱泼斯坦-巴尔病毒衣壳和核抗原(EBNA)抗体的人血清中检测到了抗HUPNA抗体。类风湿性关节炎血清中抗EBNA和抗HUPNA抗体的发生率较高。