Trocha P, D'Amato N A
Clin Chem. 1978 Feb;24(2):193-9.
Numerous methods are available for measuring urinary steroids in evaluating endocrine dysfunctions. Measurements of these particular steroids, or groups of them, usually involve tedious isolation methods, corrections for interferences and losses of the steroid, and (or) expensive reagents. We show how gas-liquid chromatography provides a rapid, sensitive, and direct method for several steroid metabolites in urine. Androstanol, androsterone, etiocholanolone, dehydroisoandrosterone, pregnanediol, pregnanetriol, 11-keto-17-ketosteroids, and the 11beta-hydroxy-17-ketosteroids can be identified and quantified.
在评估内分泌功能障碍时，有多种方法可用于测量尿类固醇。对这些特定类固醇或其组群的测量通常涉及繁琐的分离方法、对类固醇干扰和损失的校正，以及（或）昂贵的试剂。我们展示了气液色谱法如何为尿液中的几种类固醇代谢物提供一种快速、灵敏且直接的方法。雄烷醇、雄酮、本胆烷醇酮、脱氢异雄酮、孕二醇、孕三醇、11-酮-17-酮类固醇以及11β-羟基-17-酮类固醇均可被识别和定量。
