Fudge A N, Fraser C G
Clin Chem. 1978 Feb;24(2):374-6.
We describe an evaluation of the "RES-OMAT FE-59" radiometric kit (Mallinckrodt Australia Pty. Ltd.) for determination of total iron-binding capacity and unsaturated iron-binding capacity; serum iron may be calculated from total iron-binding capacity and unsaturated iron-binding capacity. Values obtained on patients' sera were compared with those obtained by continuous-flow analysis; correlation coefficients were 0.92, 0.93, and 0.88 for total iron-binding capacity, unsaturated iron-binding capacity, and iron, respectively. Precision between-run and within-run was almost identical and compares very favorably with that reported by Dixon [Ann. Clin. Biochem. 10, 127 (1973)] and that achieved in inter-laboratory surveys. Analytical recovery averaged only 83.5%, but results of analyses of sera with assigned or consensus values showed good accuracy. Satisfactory performance cannot be achieved with lyophilzed bovine quality-control materials. The kit has significant technical advantages over colorimetric methods and good short- and long-term analytical performance.
我们描述了对用于测定总铁结合力和不饱和铁结合力的“RES-OMAT FE-59”放射性试剂盒（澳大利亚马林克罗特私人有限公司）的评估；血清铁可由总铁结合力和不饱和铁结合力计算得出。将患者血清的检测值与连续流动分析法所得结果进行比较；总铁结合力、不饱和铁结合力和铁的相关系数分别为0.92、0.93和0.88。批间精密度和批内精密度几乎相同，与迪克森[《临床生物化学年鉴》10, 127 (1973)]报道的以及实验室间调查所达到的精密度相比非常有利。分析回收率平均仅为83.5%，但对具有指定值或共识值的血清分析结果显示出良好的准确性。冻干牛质量控制材料无法实现令人满意的性能。该试剂盒与比色法相比具有显著的技术优势，且具有良好的短期和长期分析性能。
