Mather J P, Saez J M, Haour F
Steroids. 1981 Jul;38(1):35-44. doi: 10.1016/0039-128x(81)90019-2.
Primary cultures of interstitial cells were prepared from the testis of mice, rats, and pigs. The cells were grown in a defined medium supplemented with low (0.1%) serum and insulin, transferrin and epidermal growth factor. Comparisons of the interstitial cell cultures from the three species were made for plating efficiency, cell survival, maintenance of hCG receptors and maintenance of steroidogenic responsiveness to hCG. The porcine cultures had a higher plating efficiency and higher hCG receptor levels per cell than Leydig cells from either rodent. Additionally, the porcine cells showed an increase in testosterone (T) production with hCG stimulation throughout their lifespan in culture while the rodent cultures showed a decrease in T stimulation with time with no stimulation by day 6 in culture. These data indicate that species differences exist in hCG receptor concentrations per cell, the maintenance of hCG receptors and steroidogenic response in culture. The initial high survival, purity and continued functional response of porcine interstitial cell cultures make them a superior system for the study of gonadotropin regulation of Leydig cell function.
间质细胞的原代培养物取自小鼠、大鼠和猪的睾丸。细胞在添加了低浓度（0.1%）血清以及胰岛素、转铁蛋白和表皮生长因子的限定培养基中生长。对来自这三个物种的间质细胞培养物进行了接种效率、细胞存活、人绒毛膜促性腺激素（hCG）受体的维持以及对hCG的类固醇生成反应性的比较。猪的培养物比来自任何一种啮齿动物的睾丸间质细胞具有更高的接种效率和更高的每细胞hCG受体水平。此外，在整个培养寿命期间，猪细胞在hCG刺激下睾酮（T）产量增加，而啮齿动物培养物随着时间推移T刺激减少，在培养第6天时无刺激作用。这些数据表明，每细胞hCG受体浓度、hCG受体的维持以及培养中的类固醇生成反应存在物种差异。猪间质细胞培养物最初的高存活率、纯度和持续的功能反应使其成为研究促性腺激素对睾丸间质细胞功能调节的优越系统。
