Suzuki I, Kwok S C
Can J Biochem. 1981 Jul;59(7):484-8. doi: 10.1139/o81-067.
The cell-free ammonia-oxidizing system of Nitrosomonas europaea was resolved into three major fractions: a membrane fraction containing cytochrome a1 and c-type cytochromes, a fraction with hydroxylamine-cytochrome c reductase and a cytochrome c fraction. The ammonia-oxidizing activity was reconstituted by the combination of these three fractions. The activity was more consistently reconstituted by adding Nitrosomonas cytochrome c554 to the membrane fraction. The hydroxylamine-cytochrome c reductase activity of the membrane fraction increased with the addition of cytochrome c554, but the oxidation of hydroxylamine to nitrite required a further addition of cytochrome c552. The ammonia oxidation by the membrane plus cytochrome c554 was affected by the concentration of phosphate and the addition of bovine serum albumin, spermine, or MgCl2.
一个含有细胞色素a1和c型细胞色素的膜部分、一个含有羟胺-细胞色素c还原酶的部分以及一个细胞色素c部分。这三个部分组合后可重建氨氧化活性。向膜部分添加欧洲亚硝化单胞菌细胞色素c554能更稳定地重建该活性。添加细胞色素c554后，膜部分的羟胺-细胞色素c还原酶活性增加，但将羟胺氧化为亚硝酸盐还需要进一步添加细胞色素c552。膜部分加细胞色素c554的氨氧化受到磷酸盐浓度以及牛血清白蛋白、精胺或MgCl2添加的影响。
