Günther E, Mikolajczyk M, Schuster H
J Biol Chem. 1981 Dec 10;256(23):11970-3.
The effect of adenine ribonucleotides on the stability of Escherichia coli dnaB protein in cellular crude extracts was studied. Stabilization of dnaB protein by ATP or ADP, but not by AMP, was manifested in that (i) the activity and yield of wild type dnaB protein is enhanced in the presence of ATP, (ii) the dnaB protein of E. coli dnaB mutants, such as groPB and dnaB252/ColE1::dnaC+, which is inactive in a dnaB complementation assay, can be isolated in active form in the presence of ATP or aDP, (iii) ATP or ADP protect the dnaB protein of an E. coli dnaBts mutant from inactivation at 37 degrees C, and (iv) inactive groPB and dnaBts protein can be reactivated partially by ATP. Thus, the stabilizing effect of ATP and ADP can be exploited for the isolated of otherwise inactive or labile mutant dnaB proteins.
研究了腺嘌呤核糖核苷酸对细胞粗提物中大肠杆菌dnaB蛋白稳定性的影响。ATP或ADP对dnaB蛋白具有稳定作用，而AMP则没有，具体表现为：（i）在ATP存在的情况下，野生型dnaB蛋白的活性和产量会提高；（ii）大肠杆菌dnaB突变体（如groPB和dnaB252/ColE1::dnaC+）的dnaB蛋白，在dnaB互补试验中无活性，但在ATP或ADP存在的情况下，可以以活性形式分离出来；（iii）ATP或ADP可保护大肠杆菌dnaBts突变体的dnaB蛋白在37℃下不被灭活；（iv）无活性的groPB和dnaBts蛋白可被ATP部分重新激活。因此，ATP和ADP的稳定作用可用于分离原本无活性或不稳定的突变dnaB蛋白。
