Schmidt F J, Thompson J, Lee K, Dijk J, Cundliffe E
J Biol Chem. 1981 Dec 10;256(23):12301-5.
Ribosomal protein L11 of Escherichia coli was bound to 23 S rRNA and the resultant complex was digested with ribonuclease T1. A single RNA fragment, protected by protein L11, was isolated from such digests and was shown to rebind specifically to protein L11. The nucleotide sequence of this RNA fragment was examined by two-dimensional fingerprinting of ribonuclease digests. It proved to be 61 residues long and the constituent oligonucleotides could be fitted perfectly between residues 1052 and 1112 of the nucleotide sequence of E. coli 23 S rRNA.
将大肠杆菌的核糖体蛋白L11与23 S rRNA结合，然后用核糖核酸酶T1消化所得复合物。从这些消化产物中分离出一个受蛋白L11保护的单一RNA片段，并证明其能特异性地重新结合到蛋白L11上。通过核糖核酸酶消化产物的二维指纹图谱分析了该RNA片段的核苷酸序列。结果表明它有61个残基长，其组成的寡核苷酸能完美地对应于大肠杆菌23 S rRNA核苷酸序列中第1052至1112位的残基之间。
